Composite

Part:BBa_K3286205:Design

Designed by: Ben Kuipers   Group: iGEM19_Wageningen_UR   (2019-10-20)


Vc2 Riboswitch + sfGFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 392
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

A high level of Vc2 riboswitch in the cell might cause some Vc2 Riboswitches not being able to be bind c-di-GMP molecules since these might get scarce. Therefore we used the mid-strength Anderson promoter to test this system. The RBS is already present in the Vc2 Riboswitch sequence.


Source

These source of the part will be its components.

References